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KMID : 0881720200350060574
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Journal of Food Hygiene and Safety
2020 Volume.35 No. 6 p.574 ~ p.580
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Food Fraud Monitoring of Commercial Sciaenidae Seafood Product Using DNA Barcode Information
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Park Eun-Ji
Jo Ah-Hyeon
Kang Ju-Young
Lee Han-Cheol
Park Min-Ji
Yang Ji-Young
Shin Ji-Young
Kim Kun-Do
Kim Jong-Oh
Seo Yong-Bae
Kim Jong-Beom
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Abstract
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In this study we sought to determine the food fraud by discriminating species of commercial seafoodproduct such as Larimichthys polyactis, Larimichthys crocea, Pennahia argentatus, and Miichthys miiuy, whichare difficult to morphologically discriminate. After amplifying the mitochondrial cytochrome c oxidase subunit I geneof the reference fish, the DNA sequences of the amplified PCR products were analyzed. As a result, a 655 bp sequencefor species identification was selected for use as DNA barcodes. To confirm the DNA data and primer set, the DNAbarcode sequence of each fish was compared to that in that in the NCBI. All of the DNA barcode data were matchedwith the gene sequence of each fish in the NCBI. A total of 32 processed seafood products (8 L. polyactis, 12 L.
crocea, 3 Pennahia argentatus, and 9 Miichthys miiuy) were investigated. Homology of 97% or more in DNAsequences was judged as the same species. As a result of the monitoring, there were no discovered cases of forgery oralteration. However, the use of a raw material name having no matching standard name in the Korea Food Code maycause consumer confusion. Therefore, it is suggested that the standard name or scientific name be co-labeled with theraw material name on seafood products to prevent consumer confusion.
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KEYWORD
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Seafood product, DNA barcode, Food fraud, Species identification
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